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miR-26b、miR-30e 在老年性痴呆症患者 血清中的表达及临床意义
梁昌权凌侬喜林小珍谢建春唐京雄吴锦华
()
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目的 研究 miR-26b、miR-30e 在老年性痴呆患者血清中的表达特点和临床意义。 方法 选取 2015 年 6 月至 2017 年 9 月江门市新会区第三人民医院收治的 200 例老年痴呆症患者进行研 究,其中阿尔茨海默病患者100例为AD组,轻度认知功能障碍(MCI)患者100例为MCI组,另选取100例 同期在本院的健康体检者为 NC 组,通过实时荧光定量 PCR(qRT-PCR)检测各组血清中 miR-26b、miR- 30e 的表达水平,比较三组之间一般临床资料以及甘油三酯(TG)、总胆固醇(TC)、低密度脂蛋白胆固醇 (LDL-C)、高密度脂蛋白胆固醇(HDL-C)、同型半胱氨酸(Hcy)、脂联素(ANP)含量;Pearson 相关分析 AD 患者血清 miR-26b、miR-30e 与各生化指标的相关性;二分类 Logistic 回归分析影响 AD 发生的危险因素。 结果 相对于 NC 组,AD 组、MCI 组血清中 Hcy、LDL-C 含量显著升高,ANP 含量显著降低(P< 0.05);AD 组、MCI 组血清中 miR-26b 表达水平分别为 0.51(0.15,0.86)、0.79(0.21,1.14)显著低于 NC 组[1.06(0.42, 1.32)](P< 0.05),AD 组血清中 miR-26b 表达水平明显低于 MCI 组(P< 0.05);AD 组、MCI 组血清中 miR- 30e 表 达 水 平 分 别 为 1.27(0.53,1.72)、1.02(0.35,1.65),明 显 高 于 NC 组[0.86(0.43,1.32)](P< 0.05), AD 组血清中 miR-30e 表达水平明显高于 MCI 组(P< 0.05)。miR-26b 相对表达水平与 ANP 值呈正相关 (r=0.626,P< 0.05),与 LDL-C、Hcy 值呈负相关(r=-0.517、-0.523,P< 0.05);miR-30e 表达水平与 LDL-C、 Hcy 值呈正相关(r=0.536、0.644,P< 0.05),与 ANP 值、miR-26b 表达水平呈负相关(r=-0.732、-0.563, P< 0.05)。Logistic 回归分析发现,Hcy、LDL-C、miR-26b、miR-30e 是 AD 发生的独立危险因素(P< 0.05), ANP 是 AD 的保护因素(P< 0.05)。结论 AD 患者血清中 miR-26b 表达显著下调,miR-30e 表达水平显 著上调,miR-26b、miR-30e 二者均与 AD 的发生发展有关。
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Expressions and clinical significance of miR-26b and miR-30e in serum of patients with Alzheimer disease
Liang Changquan, Ling Nongxi, Lin Xiaozhen, Xie Jianchun, Tang Jingxiong, Wu Jinhua
()
Abstract:
Objective To study the expression characteristics and clinical significance of miR-26b and miR-30e in serum of patients with Alzheimer disease (AD). Methods A total of 200 patients with senile dementia admitted to our hospital from June 2015 to September 2017 were selected for the study. Among them, 100 patients with AD were assigned as AD group, the other 100 patients with mild cognitive impairment (MCI) were assigned as MCI group. Another 100 patients who took health examination in our hospital during the same period were assigned as NC group. Real-time fluorescence quantitative polymerase chain reaction (qRT-PCR) was used to detect the expression levels of miR-26b and miR-30e in serum of each group. The general clinical data and the contents of triglyceride (TG), total cholesterol (TC), low density lipoprotein cholesterol (LDL-C), high density lipoprotein cholesterol (HDL-C), homocysteine (Hcy) and adiponectin (ANP) were compared among the three groups. Pearson correlation analysis was used to analyze the correlations between serum miR-26b, miR-30e and biochemical parameters in AD patients. The risk factors of AD were analyzed by binary logistic regression. Results Compared with NC group, the levels of serum Hcy and LDL-C in AD group and MCI group increased significantly, while the level of ANP decreased significantly (P < 0.05). The levels of serum miR-26b in AD group and MCI group were 0.51 (0.15,0.86) and 0.79 (0.21,1.14) respectively, which were significantly lower than that in NC group 1.06 (0.42, 1.32) (P< 0.05). The levels of serum miR-26b in AD group were significantly lower than that of MCI group (P<0.05). The level of serum miR-30e in AD and MCI groups were 1.27 (0.53,1.72) and 1.02 (0.35,1.65), which were significantly lower than those in NC group 0.86 (0.43,1.32) (P< 0.05). The serum level of miR-30e in AD group was significantly higher than that in MCI group (P< 0.05). The expression level of miR-26b was positively correlated with ANP (r=0.626,P< 0.05), and negatively correlated with LDL-C and Hcy (r=-0.517,-0.523,P< 0.05). The expression level of miR-30e was positively correlated with LDL-C and Hcy values (r=0.536,0.644,P < 0.05), while negatively correlated with ANP values and the expression level of miR-26b (r=-0.732,-0.563,P < 0.05). Logistic regression analysis showed that Hcy, LDL-C, miR- 26b and miR-30e were independent risk factors for AD (P < 0.05). And ANP was protective factor for AD (P < 0.05). Conclusions The expression of miR-26b in serum of AD patients is significantly down-regulated, the expression of miR-30e is significantly up-regulated. Both miR-26b and miR-30e are related to the occurrence and development of AD.

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